Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ciliobrevin D 是细胞渗透性,可逆和特异性的 AAA + ATPase 运动细胞质动力蛋白抑制剂。它在体外抑制依赖于动力蛋白的微管滑动和 ATPase 活性。它抑制Hedgehog 信号和初级纤毛形成。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 528 | 现货 | ||
2 mg | ¥ 778 | 现货 | ||
5 mg | ¥ 1,290 | 现货 | ||
10 mg | ¥ 1,980 | 现货 | ||
25 mg | ¥ 3,970 | 现货 | ||
50 mg | ¥ 5,680 | 现货 | ||
100 mg | ¥ 7,910 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,390 | 现货 |
产品描述 | Ciliobrevin D is a AAA+ ATPase motor cytoplasmic dynein inhibitor. Ciliobrevin D inhibits Hedgehog (Hh) signaling and primary cilia formation and it also inhibits dynein-dependent microtubule gliding and ATPase activity in vitro. |
体外活性 | Ciliobrevin D reversibly inhibits melanosome aggregation, however, the non-cilia-disrupting derivative had no discernible effect at comparable doses. Cells treated with Ciliobrevin D exhibits abnormal (unfocused, multipolar, or collapsed) spindles with disrupted γ-tubulin localization in NIH-3T3 cells. Ciliobrevin D addition also reversibly disrupts the pre-formed spindles of metaphase-arrested cells and reduces overall microtubule levels and it similarly abrogates the movement of peroxisomes in Drosophila S2 cells[1]. |
体内活性 | In the testis in vivo, Knockdown of Dync1h1 or inactivation of dynein 1 by Ciliobrevin D perturbs spermatogenesis. The use of Ciliobrevin D to inactivate dynein 1 in the testis in vivo perturbs MT organization through changes in the spatial expression of EB1, perturbs F-actin organization, and perturbs distribution of adhesion protein complexes at the BTB, leading to a loss of BTB integrity[3]. |
分子量 | 392.62 |
分子式 | C17H8Cl3N3O2 |
CAS No. | 1370554-01-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 5 mg/mL (12.73 mM), Sonication and heating to 80℃ are recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.547 mL | 12.735 mL | 25.4699 mL | 63.6748 mL |
5 mM | 0.5094 mL | 2.547 mL | 5.094 mL | 12.735 mL | |
10 mM | 0.2547 mL | 1.2735 mL | 2.547 mL | 6.3675 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Ciliobrevin D 1370554-01-0 GPCR/G Protein Membrane transporter/Ion channel Stem Cells ATPase Hedgehog/Smoothened inhibit melanosome spindles cytoplasmic γ-tubulin Inhibitor dynein ATPases Hedgehog peroxisomes aggregation inhibitor